# Developmental Stage Classification of Embryos Using Two-Stream Neural Network with Linear-Chain Conditional Random Field Source: [Developmental Stage Classification of Embryos Using Two-Stream Neural Network with Linear-Chain Conditional Random Field]() Collection index: [llms.txt]() Data updated: 2025-12-24T15:58:00.237Z Authors: Stanislav Lukyanenko, Won-Dong Jang, Donglai Wei, Robbert Struyven, Yoon Kim, Brian Leahy, Helen Yang, Alexander Rush, Dalit Ben-Yosef, Daniel Needleman, Hanspeter Pfister Published: 2021-07-13T19:56:01.000Z ## Abstract The developmental process of embryos follows a monotonic order. An embryo can progressively cleave from one cell to multiple cells and finally transform to morula and blastocyst. For time-lapse videos of embryos, most existing developmental stage classification methods conduct per-frame predictions using an image frame at each time step. However, classification using only images suffers from overlapping between cells and imbalance between stages. Temporal information can be valuable in addressing this problem by capturing movements between neighboring frames. In this work, we propose a two-stream model for developmental stage classification. Unlike previous methods, our two-stream model accepts both temporal and image information. We develop a linear-chain conditional random field (CRF) on top of neural network features extracted from the temporal and image streams to make use of both modalities. The linear-chain CRF formulation enables tractable training of global sequential models over multiple frames while also making it possible to inject monotonic development order constraints into the learning process explicitly. We demonstrate our algorithm on two time-lapse embryo video datasets: i) mouse and ii) human embryo datasets. Our method achieves 98.1 % and 80.6 % for mouse and human embryo stage classification, respectively. Our approach will enable more profound clinical and biological studies and suggests a new direction for developmental stage classification by utilizing temporal information. ## Recorded benchmark results A research result applies to its stated dataset, split, metric and evaluation configuration. Compare like-for-like settings, preserve the metric's unit and direction, and distinguish paper-reported results from independent reproduction. The best result recorded here is not a claim of exhaustive, current global coverage. - [Developmental Stage Classification on Human embryo dataset (test)](): Developmental Stage Classification; Human embryo dataset (test); Global Accuracy: 80.6. - [Embryo Stage Classification on NYU Mouse Embryo 2 (test)](): Embryo Stage Classification; NYU Mouse Embryo 2 (test); Global Accuracy: 0.981. ## Original sources and implementation [Original paper]() [PDF]()