# Advection-enhanced kinetics in microtiter plates for improved surface assay quantitation and multiplexing capabilities Source: [Advection-enhanced kinetics in microtiter plates for improved surface assay quantitation and multiplexing capabilities]() Collection index: [llms.txt]() Data updated: 2025-10-28T13:56:21.317Z Authors: Iago Pereiro, Anna Fomitcheva Khartchenko, Robert D. Lovchik, Govind V. Kaigala Published: 2022-02-07T11:27:29.000Z ## Abstract Surface assays such as ELISA are pervasive in clinics and research and predominantly standardized in microtiter plates (MTP). MTPs provide many advantages but are often detrimental to surface assay efficiency due to inherent mass transport limitations. Microscale flows can overcome these and largely improve assay kinetics. However, the disruptive nature of microfluidics with existing labware and protocols has narrowed its transformative potential. We present WellProbe, a novel microfluidic concept compatible with MTPs. With it, we show that immunoassays become more sensitive at low concentrations (up to 9-fold signal improvement in 12-fold less time), richer in information with 3-4 different kinetic conditions, and can be used to estimate kinetic parameters, minimize washing steps and non-specific binding, and identify compromised results. We further multiplex single-well assays combining WellProbes kinetic regions with tailored microarrays. Finally, we demonstrate our system in a context of immunoglobulin subclass evaluation, increasingly regarded as clinically relevant. ## Recorded benchmark results A research result applies to its stated dataset, split, metric and evaluation configuration. Compare like-for-like settings, preserve the metric's unit and direction, and distinguish paper-reported results from independent reproduction. The best result recorded here is not a claim of exhaustive, current global coverage. ## Original sources and implementation [Original paper]() [PDF]()