# On the quasi-steady-state assumption in enzyme kinetics: rigorous analysis

Source: [On the quasi-steady-state assumption in enzyme kinetics: rigorous analysis](<https://www.sota2.com/research/paper/68fa588b1e209131ce9af320>)

Collection index: [llms.txt](<https://www.sota2.com/research/llms.txt>)

Data updated: 2025-10-25T15:23:38.191Z

Authors: Vasiliki Bitsouni, Nikolaos Gialelis, Ioannis G.Stratis

Published: 2021-09-29T19:01:40.000Z

## Abstract

We study, from a purely quantitative point of view, the quasi-steady-state assumption for the fundamental mathematical model of the general enzymatic reaction: we re-establish, on a rigorous basis, certain already known results and we propose a novel approach to the subject, that makes waivable an ambiguous, widely used, practice. In particular, we define the two parts of the assumption in a quantitative fashion, we employ a simple algorithm for the proper scaling of the corresponding problem which naturally provides us with the necessary and sufficient information, and we comment, among other issues, on a dispensable third part of the assumption.

## Recorded benchmark results

A research result applies to its stated dataset, split, metric and evaluation configuration. Compare like-for-like settings, preserve the metric's unit and direction, and distinguish paper-reported results from independent reproduction. The best result recorded here is not a claim of exhaustive, current global coverage.

## Original sources and implementation

[Original paper](<https://arxiv.org/abs/2109.14667>)

[PDF](<https://arxiv.org/pdf/2109.14667.pdf>)