# Holographic immunoassays

Source: [Holographic immunoassays](<https://www.sota2.com/research/paper/68fa3c711e209131ce8da823>)

Collection index: [llms.txt](<https://www.sota2.com/research/llms.txt>)

Data updated: 2025-10-27T04:04:17.802Z

Authors: Kaitlynn Snyder, Rushna Quddus, Andrew D. Hollingsworth, Kent Kirshenbaum, David G. Grier

Published: 2020-07-18T15:03:08.000Z

## Abstract

The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) as they attach to micrometer-diameter colloidal beads coated with protein A. These measurements yield the antibodies' binding rates and can be inverted to obtain the concentration of antibodies in solution. Holographic molecular binding assays therefore can be used to perform fast quantitative immunoassays that are complementary to conventional serological tests.

## Recorded benchmark results

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## Original sources and implementation

[Original paper](<https://arxiv.org/abs/2007.09447>)

[PDF](<https://arxiv.org/pdf/2007.09447.pdf>)