# Kinetics of actin networks formation measured by time resolved particle-tracking microrheology Source: [Kinetics of actin networks formation measured by time resolved particle-tracking microrheology]() Collection index: [llms.txt]() Data updated: 2025-10-25T00:41:58.613Z Authors: Maayan Levin, Raya Sorkin, David Pine, Rony Granek, Anne Bernheim-Groswasser, Yael Roichman Published: 2020-03-16T06:52:21.000Z ## Abstract Actin is one of the most studied cytoskeleton proteins showing a very rich span of structures. It can self-assemble actively into dynamical structures that govern the mechanical properties of the cell, its motility and its division. However, only very few studies characterize the kinetics of the active actin self-assembly process beyond the formation of an entangled network. Here, we follow actin polymerization kinetics and organization into entangled networks using time resolved passive microrheology. We establish a relationship between the initial concentration of monomers, the active polymerization and network formation kinetics, and the viscoelastic properties from the onset of actin polymerization upto the formation of a steady state entangled network. Surprisingly, we find that at high enough initial monomer concentrations the elastic modulus of the forming actin networks overshoots and then relaxes with a -2/5 power law, that we attribute to rearrangements of the network into a steady state structure. ## Recorded benchmark results A research result applies to its stated dataset, split, metric and evaluation configuration. Compare like-for-like settings, preserve the metric's unit and direction, and distinguish paper-reported results from independent reproduction. The best result recorded here is not a claim of exhaustive, current global coverage. ## Original sources and implementation [Original paper]() [PDF]()