# A generalized kinetic framework applied to whole-cell catalysis in biofilm flow reactors clarifies performance enhancements

Source: [A generalized kinetic framework applied to whole-cell catalysis in biofilm flow reactors clarifies performance enhancements](<https://www.sota2.com/research/paper/68fa398a1e209131ce8c3657>)

Collection index: [llms.txt](<https://www.sota2.com/research/llms.txt>)

Data updated: 2025-10-24T23:34:13.006Z

Authors: Mir Pouyan Zarabadi, Manon Couture, Steve J. Charette, Jesse Greener

Published: 2019-04-10T19:56:15.000Z

## Abstract

A common kinetic framework for studies of whole-cell catalysis is vital for understanding and optimizing bioflow reactors. In this work, we demonstrate the applicability of a flow-adapted version of Michaelis-Menten kinetics to a catalytic bacterial biofilm. A three-electrode microfluidic electrochemical flow cell measured increased turnover rates by as much as 50% from a Geobacter sulfurreducens biofilm as flow rate was varied. Based on parameters from the applied kinetic framework, flow-induced increases to turnover rate, catalytic efficiency and device reaction capacity could be linked to an increase in catalytic biomass. This study demonstrates that a standardized kinetic framework is critical for quantitative measurements of new living catalytic systems in flow cells and for benchmarking against well-studied catalytic systems such as enzymes.

## Recorded benchmark results

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## Original sources and implementation

[Original paper](<https://arxiv.org/abs/1904.05412>)

[PDF](<https://arxiv.org/pdf/1904.05412.pdf>)