# Questioning the reliability of estimates of enzyme inhibitor constant: Case of competitive inhibition

Source: [Questioning the reliability of estimates of enzyme inhibitor constant: Case of competitive inhibition](<https://www.sota2.com/research/paper/68fa34991e209131ce89a587>)

Collection index: [llms.txt](<https://www.sota2.com/research/llms.txt>)

Data updated: 2025-10-24T20:45:45.009Z

Authors: Sharmistha Dhatt

Published: 2016-10-18T06:39:00.000Z

## Abstract

Reliability of kinetic parameters are crucial in understanding enzyme kinetics within cellular system. The present study suggests a few cautions that need introspection for estimation of parameters like K(M), V(max) and K(I) using Lineweaver-Burk plots. The quality of IC(50) too needs a thorough reinvestigation because of its direct link with K(I) and K(M) values. Inhibition kinetics under both steady-state and non-steady-state conditions are studied and errors in estimated parameters are compared against actual values to settle the question of their adequacy.

## Recorded benchmark results

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## Original sources and implementation

[Original paper](<https://arxiv.org/abs/1610.05451>)

[PDF](<https://arxiv.org/pdf/1610.05451.pdf>)