# A Biochemical Logic Approach to Biomarker-Activated Drug Release

Source: [A Biochemical Logic Approach to Biomarker-Activated Drug Release](<https://www.sota2.com/research/paper/68fa2c291e209131ce84da18>)

Collection index: [llms.txt](<https://www.sota2.com/research/llms.txt>)

Data updated: 2025-10-28T13:53:16.615Z

Authors: V. Bocharova, O. Zavalov, K. MacVittie, M. A. Arugula, N. V. Guz, M. E. Dokukin, J. Halamek, I. Sokolov, V. Privman, E. Katz

Published: 2013-04-30T13:53:10.000Z

## Abstract

The present study aims at integrating drug-releasing materials with signal-processing biocomputing systems. Enzymes alanine transaminase (ALT) and aspartate transaminase (AST)---biomarkers for liver injury---were logically processed by a biocatalytic cascade realizing Boolean AND gate. Citrate produced in the system was used to trigger a drug-mimicking release from alginate microspheres. In order to differentiate low vs. high concentration signals, the microspheres were coated with a protective shell composed of layer-by-layer adsorbed poly(L-lysine) and alginate. The alginate core of the microspheres was prepared from (Fe3+)-cross-linked alginate loaded with rhodamine 6G dye mimicking a drug. Dye release from the core occurred only when both biomarkers, ALT and AST, appeared at their high pathophysiological concentrations jointly indicative of liver injury. The signal-triggered response was studied at the level of a single microsphere, yielding information on the dye release kinetics.

## Recorded benchmark results

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## Original sources and implementation

[Original paper](<https://arxiv.org/abs/1304.8000>)

[PDF](<https://arxiv.org/pdf/1304.8000.pdf>)