# Unzipping Kinetics of Double-Stranded DNA in a Nanopore

Source: [Unzipping Kinetics of Double-Stranded DNA in a Nanopore](<https://www.sota2.com/research/paper/68fa25c02c2a3760193b371d>)

Collection index: [llms.txt](<https://www.sota2.com/research/llms.txt>)

Data updated: 2025-10-24T10:56:31.190Z

Authors: Alexis F. Sauer-Budge, Jacqueline A. Nyamwanda, David K. Lubensky, Daniel Branton

Published: 2002-09-17T20:09:33.000Z

## Abstract

We studied the unzipping kinetics of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. PCR analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.

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## Original sources and implementation

[Original paper](<https://arxiv.org/abs/cond-mat/0209414>)

[PDF](<https://arxiv.org/pdf/cond-mat/0209414.pdf>)